Summary
A cDNA coding for the carboxy-terminal region of human protein S and containing a
complete 3’-untranslated region, was isolated by a combination of antibody screening
of a λgt11 human liver cDNA expression library and in situ hybridization of a pUC9
human liver cDNA library.
Hybridization analysis of human total DNA with radiolabelled non-overlapping cDNA
restriction fragments revealed the existence of two genes per haploid genome homologous
with the protein S cDNA. Both genes were mapped to chromosome 3 using human-rodent
cell hybrids. Neither of the genes showed polymorphism for sixteen different enzymes
upon hybridization with the protein S cDNA.
Keywords
Protein S - Gene - Chromosome - RFLP - Thrombosis